Complete plastome phylogeny and an update on cox1 intron evolution of Hyoscyameae (Solanaceae)

Within the family Solanaceae, tribe Hyoscyameae comprises eight genera distributed across Eurasia. Despite a few attempts to understand relationships within this tribe, affiliations among most remain unresolved. Recently, complete chloroplast genomes from several species of were published, enabling phylogenomic inferences. We sequenced plastome Scopolia carniolica, second genus be reported. Genomic comparisons revealed identical gene content and small differences in genome length. Phylogenetic analyses confirmed that Atropa is an early-diverging lineage sister rest resolved as two clades. One includes well-supported between Przewalskia, Physochlaina, Scopolia, these three are Atropanthe. This clade composed Hyoscyamus Anisodus. The strongly supported phylogenetic Atropanthe, Anisodus, represent major advancements previous studies not able resolve relationships. Interestingly, paraphyletic respect based on ITS2 sequences. Finally, evolution mitochondrial cox1 intron reinterpreted. Two independent horizontal acquisitions inferred, one ancestor S. japonica another Hyoscyamus, with no losses.